Storage temp. Store at -20°C inert atmosphere (e.g., Nitrogen or Argon) in a dry place to prevent moisture-induced degradation.
Physical Form – colorless viscous liquid
Reactive Groups: Homobifunctional TFP (tetrafluorophenyl) esters at both terminals, designed for precise amine-to-amine cross-linking.
Spacer Arm: A discrete tetraethylene glycol (PEG4) chain, providing a defined distance between conjugated molecules to minimize steric hindrance.
Performance Advantages
Superior Hydrolytic Stability: Unlike traditional NHS esters, TFP esters exhibit significantly higher stability in aqueous buffers, especially at basic pH levels (up to pH 9.0). This reduces the waste of reagent due to hydrolysis and increases overall bioconjugation efficiency.
Enhanced Water Solubility: The hydrophilic PEG4 spacer improves the solubility of the cross-linker and its resulting conjugates in aqueous media, which is critical for maintaining the biological activity of proteins and antibodies.
Optimized Reaction Window: TFP esters react efficiently with primary and secondary amines at a slightly higher optimal pH (8.3–8.5) than NHS esters, providing a broader operational window for sensitive protocols.
